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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    WB analysis using 88267-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88267-1-RR (NOC2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    WB analysis using 88267-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88267-1-RR (NOC2L antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    IHC staining of human placenta using 88267-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 88267-1-RR (NOC2L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    IF Staining of U2OS using 88267-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOC2L antibody (88267-1-RR, Clone: 260756G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity of 88267-1-RR

    Affinity of 88267-1-RR

    Biolayer interferometry (BLI) kinetic assays of 88267-1-RR against Human NOC2L were performed. The affinity constant is 0.413 nM.

NOC2L Recombinant monoclonal antibody

NOC2L Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 88267-1-RR
Clone No.260756G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NIR, NOC2-like protein, Novel INHAT repressor, Nucleolar complex protein 2 homolog, Protein NOC2 homolog

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse cerebellum tissue, U2OS cells, rat cerebellum tissue, HL-60 cells, U-251 cells, HeLa cells, HT-1080 cells, PANC-1 cells, MDA-MB-453 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88267-1-RR targets NOC2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26952

Product name: Recombinant human NOC2L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-202 aa of BC003555

Sequence: MAAAGSRKRRLAELTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRKGRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDSDSSEEEEGPFHSLPDVLEEASEEEDGAEEGEDGDRVPRGLKGKKNSVPVTVAMVERWKQAAKQRLTPKLFHEVVQAFRAAVATTRGDQESAEANKFQVTDSA

相同性解析による交差性が予測される生物種
Full Name nucleolar complex associated 2 homolog (S. cerevisiae)
Calculated molecular weight 749 aa, 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC003555
Gene Symbol NOC2L
Gene ID (NCBI) 26155
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3T9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).

Protocols

Product Specific Protocols
IF protocol for NOC2L antibody 88267-1-RRDownload protocol
IHC protocol for NOC2L antibody 88267-1-RRDownload protocol
WB protocol for NOC2L antibody 88267-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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