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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGS8 Recombinant monoclonal antibody (88102-1-RR)

    WB analysis using 88102-1-RR (same clone as 88102-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88102-1-RR (RGS8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using RGS8 Recombinant monoclonal antibody (88102-1-RR)

    WB analysis of pig brain using 88102-1-RR (same clone as 88102-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 88102-1-RR (RGS8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using RGS8 Recombinant monoclonal antibody (88102-1-RR)

    WB analysis of rat liver using 88102-1-RR (same clone as 88102-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 88102-1-RR (RGS8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03353-2

    Sandwich ELISA sample test of MP03353-2

    The mean RGS8 concentration was determined to be 88.79 ng/mL in mouse liver extract based on a 8.40 mg/mL extract load, 4.65 ng/mL in mouse skin extract based on a 0.90 mg/mL extract load and 41.22 ng/mL in rat brain extract based on a 3.90 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03353-2

    Sandwich ELISA standard curve of MP03353-2

    Sandwich ELISA standard curve of MP03353-2, Human RGS8 Recombinant Matched Antibody Pair - PBS only. 88102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26535. 88102-3-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RGS8 Recombinant monoclonal antibody (88102-1-RR)

    IF Staining of mouse cerebellum using 88102-1-RR (same clone as 88102-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RGS8 antibody (88102-1-RR, Clone: 260122C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RGS8 Recombinant monoclonal antibody (88102-1-RR)

    IF Staining of mouse cerebellum using 88102-1-RR (same clone as 88102-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RGS8 antibody (88102-1-RR, Clone: 260122C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88102-1-PBS in a different storage buffer formulation.

RGS8 Recombinant monoclonal antibody, PBS Only (Capture)

RGS8 Uni-rAb® Recombinant Antibody for WB, IF-P, Sandwich ELISA, Indirect ELISA
Cat No. 88102-1-PBS
Clone No.260122C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, Sandwich ELISA, Indirect ELISA

Synonyms

Regulator of G-protein signaling 8

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

88102-1-PBS targets RGS8 as part of a matched antibody pair:

MP03353-2: 88102-1-PBS capture and 88102-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26535

Product name: Recombinant human RGS8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-198 aa of BC069718

Sequence: NKGMRTRLGCLSHKSDSCSDFTAILPDKPNRALKRLSTEEATRWADSFDVLLSHKYGVAAFRAFLKTEFSEENLEFWLACEEFKKTRSTAKLVSKAHRIFEEFVDVQAPREVNIDFQTREATRKNLQEPSLTCFDQAQGKVHSLMEKDSYPRFLRSKMYLDLLSQSQRRLS

相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 8
Calculated molecular weight 198 aa, 23 kDa
Observed molecular weight18-20 kDa
GenBank accession numberBC069718
Gene Symbol RGS8
Gene ID (NCBI) 85397
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP57771
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RGS8 is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation.

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