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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGS8 Recombinant monoclonal antibody (88102-1-RR)

    WB analysis using 88102-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88102-1-RR (RGS8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using RGS8 Recombinant monoclonal antibody (88102-1-RR)

    WB analysis of pig brain using 88102-1-RR

    pig brain tissue were subjected to SDS PAGE followed by western blot with 88102-1-RR (RGS8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using RGS8 Recombinant monoclonal antibody (88102-1-RR)

    WB analysis of rat liver using 88102-1-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 88102-1-RR (RGS8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RGS8 Recombinant monoclonal antibody (88102-1-RR)

    IF Staining of mouse cerebellum using 88102-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RGS8 antibody (88102-1-RR, Clone: 260122C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RGS8 Recombinant monoclonal antibody (88102-1-RR)

    IF Staining of mouse cerebellum using 88102-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RGS8 antibody (88102-1-RR, Clone: 260122C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RGS8 Recombinant monoclonal antibody

RGS8 Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 88102-1-RR
Clone No.260122C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, ELISA

Synonyms

Regulator of G-protein signaling 8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, pig brain tissue, rat liver tissue, rat brain tissue
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88102-1-RR targets RGS8 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26535

Product name: Recombinant human RGS8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-198 aa of BC069718

Sequence: NKGMRTRLGCLSHKSDSCSDFTAILPDKPNRALKRLSTEEATRWADSFDVLLSHKYGVAAFRAFLKTEFSEENLEFWLACEEFKKTRSTAKLVSKAHRIFEEFVDVQAPREVNIDFQTREATRKNLQEPSLTCFDQAQGKVHSLMEKDSYPRFLRSKMYLDLLSQSQRRLS

相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 8
Calculated molecular weight 198 aa, 23 kDa
Observed molecular weight18-20 kDa
GenBank accession numberBC069718
Gene Symbol RGS8
Gene ID (NCBI) 85397
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP57771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RGS8 is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation.

Protocols

Product Specific Protocols
IF protocol for RGS8 antibody 88102-1-RRDownload protocol
WB protocol for RGS8 antibody 88102-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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