Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87061-1-RR targets Phospho-TAU (Ser396/404) in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | microtubule-associated protein tau |
| Calculated molecular weight | 37-46, 79-81 kDa |
| Observed molecular weight | 45-60 kDa |
| GenBank accession number | BC000558 |
| Gene Symbol | TAU |
| Gene ID (NCBI) | 4137 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P10636 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tau isolated from individuals with AD is extensively phosphorylated and more than 40 sites have been reported so far. Hyperphosphorylated tau has an impaired ability to stabilize microtubules, and is capable of self-assembling into aggregates that can template normal tau into filaments, making hyperphosphorylation a clear disease-determining posttranslational modification in tauopathies.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TAU (Ser396/404) antibody 87061-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |