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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calponin Recombinant monoclonal antibody (86977-4-RR)

    WB analysis using 86977-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86977-4-RR (Calponin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human appendix tissue using Calponin Recombinant monoclonal antibody (86977-4-RR)

    IHC staining of human appendix using 86977-4-RR

    Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 86977-4-RR (Calponin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendix tissue using Calponin Recombinant monoclonal antibody (86977-4-RR)

    IHC staining of human appendix using 86977-4-RR

    Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 86977-4-RR (Calponin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Calponin Recombinant monoclonal antibody (86977-4-RR)

    IF Staining of mouse heart using 86977-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Calponin antibody (86977-4-RR, Clone: 252040C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Calponin Recombinant monoclonal antibody (86977-4-RR)

    IF Staining of mouse heart using 86977-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Calponin antibody (86977-4-RR, Clone: 252040C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 86977-4-RR

    Affinity of 86977-4-RR

    Biolayer interferometry (BLl) kinetic assays of 86977-4-RR against Human Calponin were performed. The affinity constant is 1.34 nM.

Calponin Recombinant monoclonal antibody

Calponin Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86977-4-RR
Clone No.252040C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, mouse bladder tissue, rat bladder tissue
Positive IHC detected inhuman appendix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86977-4-RR targets Calponin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag21384

Product name: Recombinant human Calponin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 225-265 aa of BC022015

Sequence: KRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPR

相同性解析による交差性が予測される生物種
Full Name calponin 1, basic, smooth muscle
Calculated molecular weight 33 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC022015
Gene Symbol Calponin
Gene ID (NCBI) 1264
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP51911
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kDa protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation.

Protocols

Product Specific Protocols
IF protocol for Calponin antibody 86977-4-RRDownload protocol
IHC protocol for Calponin antibody 86977-4-RRDownload protocol
WB protocol for Calponin antibody 86977-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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