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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MOF Recombinant monoclonal antibody (86910-3-RR)

    WB analysis using 86910-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86910-3-RR (MOF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using MOF Recombinant monoclonal antibody (86910-3-RR)

    IF Staining of HCT 116 using 86910-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using MOF antibody (86910-3-RR, Clone: 251926C5 ) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using MOF Recombinant monoclonal antibody (86910-3-RR)

    FC experiment of HepG2 using 86910-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MOF Recombinant monoclonal antibody (86910-3-RR, Clone:251926C5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 86910-3-RR

    Affinity and Kinetic Characterization of 86910-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86910-3-RR against Human MOF were performed. The affinity constant is below 1 pM.

MOF Recombinant monoclonal antibody

MOF Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 86910-3-RR
Clone No.251926C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

MYST1, EC:2.3.1.-, EC:2.3.1.48, Histone acetyltransferase KAT8, hMOF

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HCT 116 cells, OVCAR-3 cells, RAW 264.7 cells, PC-12 cells
Positive IF/ICC detected inHCT 116 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:600-1:2400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86910-3-RR targets MOF in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4802

Product name: Recombinant human MOF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 118-467 aa of BC037773

Sequence: AVQKNSEKYLSELAEQPERKITRNQKRKHDEINHVQKTYAEMDPTTAALEKEHEAITKVKYVDKIHIGNYEIDAWYFSPFPEDYGKQPKLWLCEYCLKYMKYEKSYRFHLGQCQWRQPPGKEIYRKSNISVYEVDGKDHKIYCQNLCLLAKLFLDHKTLYFDVEPFVFYILTEVDRQGAHIVGYFSKEKESPDGNNVACILTLPPYQRRGYGKFLIAFSYELSKLESTVGSPEKPLSDLGKLSYRSYWSWVLLEILRDFRGTLSIKDLSQMTSITQNDIISTLQSLNMVKYWKGQHVICVTPKLVEEHLKSAQYKKPPITGGWGAAVCRGRWGSVSMWTGRSQGLLIAVT

相同性解析による交差性が予測される生物種
Full Name MYST histone acetyltransferase 1
Calculated molecular weight 467 aa, 53 kDa
Observed molecular weight52 kDa
GenBank accession numberBC037773
Gene Symbol KAT8/MOF
Gene ID (NCBI) 84148
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9H7Z6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MOF, also known as MYST1, KAT8, is a member of the MYST acetyltransferase family, which is homologous to Drosophila males absent on the first (MOF) protein. Deletion of MYST1 in cells leads to genomic instability, spontaneous chromosomal aberration, cell cycle defects, reduced transcription of certain genes and defective DNA damage repair (PMID: 32711345). MYST1 dually localizes to the nucleus and mitochondria of human cancer cells (PMID: 37813994).

Protocols

Product Specific Protocols
FC protocol for MOF antibody 86910-3-RRDownload protocol
IF protocol for MOF antibody 86910-3-RRDownload protocol
WB protocol for MOF antibody 86910-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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