Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86235-1-RR targets Cytokeratin 16 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 16 |
| Calculated molecular weight | 473 aa, 51 kDa |
| Observed molecular weight | 48-50 kDa |
| GenBank accession number | BC039169 |
| Gene Symbol | Cytokeratin 16 |
| Gene ID (NCBI) | 3868 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08779 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytokeratin 16 is a Type I acidic keratin (classified as KRT16 in the human gene nomenclature) that pairs with Type II basic keratins (e.g., CK-6) to form intermediate filaments in epithelial cells. It is prominently expressed in stratified epithelia, particularly under conditions of stress, proliferation, or pathology. CK-16 is constitutively expressed in specialized epithelia (e.g., nail beds, hair follicles, oral mucosa) but is normally absent in healthy, quiescent skin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 16 antibody 86235-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |