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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using KLK3/PSA Recombinant antibody (84059-5-RR)

    WB analysis of LNCaP using 84059-5-RR

    LNCaP cells were subjected to SDS PAGE followed by western blot with 84059-5-RR (KLK3/PSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLK3/PSA Recombinant antibody (84059-5-RR)

    IHC staining of human prostate cancer using 84059-5-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84059-5-RR (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using KLK3/PSA Recombinant antibody (84059-5-RR)

    IF Staining of LNCaP using 84059-5-RR

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using KLK3/PSA antibody (84059-5-RR, Clone: 241263F8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using KLK3/PSA Recombinant antibody (84059-5-RR)

    FC experiment of HeLa using 84059-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug KLK3/PSA Recombinant antibody (84059-5-RR, Clone:241263F8) and PE-conjugated Goat Anti-Rabbit IgG(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84059-5-RR

    Affinity and Kinetic Characterization of 84059-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84059-5-RR against Human KLK3/PSA were performed. The affinity constant is 149 nM.

KLK3/PSA Recombinant monoclonal antibody

KLK3/PSA Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84059-5-RR
Clone No.241263F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

KLK3, 241263F8, APS, EC:3.4.21.77, Gamma-seminoprotein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inLNCaP cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inLNCaP cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84059-5-RR targets KLK3/PSA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0869

Product name: Recombinant Human KLK3/PSA protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-10*His

Domain: 18-261 aa of NM_001648.2

Sequence: APLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP

相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 3
Calculated molecular weight29 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberNM_001648.2
Gene Symbol KLK3
Gene ID (NCBI) 354
RRIDAB_3671627
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP07288-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for KLK3/PSA antibody 84059-5-RRDownload protocol
IHC protocol for KLK3/PSA antibody 84059-5-RRDownload protocol
WB protocol for KLK3/PSA antibody 84059-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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