Validation Data Gallery
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Tested Applications
| Positive WB detected in | ROS1728 cells, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83517-3-RR targets RPS11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34848 Product name: Recombinant human RPS11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-76 aa of BC007945 Sequence: MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGV 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S11 |
| Calculated molecular weight | 158 aa, 18 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC007945 |
| Gene Symbol | RPS11 |
| Gene ID (NCBI) | 6205 |
| RRID | AB_3671142 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P62280 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS11 antibody 83517-3-RR | Download protocol |
| IHC protocol for RPS11 antibody 83517-3-RR | Download protocol |
| WB protocol for RPS11 antibody 83517-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |