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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    WB analysis using 83517-3-RR (same clone as 83517-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83517-3-RR (RPS11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    IHC staining of human stomach cancer using 83517-3-RR (same clone as 83517-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    IHC staining of mouse testis using 83517-3-RR (same clone as 83517-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    IHC staining of mouse testis using 83517-3-RR (same clone as 83517-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    IF Staining of HepG2 using 83517-3-RR (same clone as 83517-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS11 antibody (83517-3-RR, Clone: 240327D11 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    FC experiment of MCF-7 using 83517-3-RR (same clone as 83517-3-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RPS11 Recombinant antibody (83517-3-RR, Clone:240327D11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83517-3-RR

    Affinity and Kinetic Characterization of 83517-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83517-3-RR against Human RPS11 were performed. The affinity constant is 5.05 nM.

RPS11 Recombinant monoclonal antibody, PBS Only

RPS11 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83517-3-PBS
Clone No.240327D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

240327D11, 40S ribosomal protein S11, ribosomal protein S11, Small ribosomal subunit protein uS17

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83517-3-PBS targets RPS11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34848

Product name: Recombinant human RPS11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-76 aa of BC007945

Sequence: MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGV

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S11
Calculated molecular weight 158 aa, 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC007945
Gene Symbol RPS11
Gene ID (NCBI) 6205
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP62280
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

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