×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    WB analysis using 83517-3-RR (same clone as 83517-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83517-3-RR (RPS11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    IHC staining of mouse testis using 83517-3-RR (same clone as 83517-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    IHC staining of mouse testis using 83517-3-RR (same clone as 83517-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    IHC staining of human stomach cancer using 83517-3-RR (same clone as 83517-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    IF Staining of HepG2 using 83517-3-RR (same clone as 83517-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS11 antibody (83517-3-RR, Clone: 240327D11 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using RPS11 Recombinant monoclonal antibody (83517-3-RR)

    FC experiment of MCF-7 using 83517-3-RR (same clone as 83517-3-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RPS11 Recombinant antibody (83517-3-RR, Clone:240327D11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83517-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83517-3-RR

    Affinity and Kinetic Characterization of 83517-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83517-3-RR against Human RPS11 were performed. The affinity constant is 5.05 nM.

RPS11 Recombinant monoclonal antibody, PBS Only

RPS11 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83517-3-PBS
Clone No.240327D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

240327D11, 40S ribosomal protein S11, ribosomal protein S11, Small ribosomal subunit protein uS17

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83517-3-PBS targets RPS11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34848

Product name: Recombinant human RPS11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-76 aa of BC007945

Sequence: MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGV

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S11
Calculated molecular weight 158 aa, 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC007945
Gene Symbol RPS11
Gene ID (NCBI) 6205
RRIDAB_3671142
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP62280
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

||
New chat

Able™

正在加载，请稍候...