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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPP30 Recombinant antibody (83481-5-RR)

    WB analysis using 83481-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83481-5-RR (RPP30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RPP30 Recombinant antibody (83481-5-RR)

    WB analysis of HEK-293 using 83481-5-RR

    WB result of RPP30 antibody (83481-5-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPP30 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RPP30 Recombinant antibody (83481-5-RR)

    IHC staining of human ovary cancer using 83481-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83481-5-RR (RPP30 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RPP30 Recombinant antibody (83481-5-RR)

    IHC staining of human ovary cancer using 83481-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83481-5-RR (RPP30 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using RPP30 Recombinant antibody (83481-5-RR)

    IF Staining of U-251 using 83481-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (83481-5-RR, Clone: 240468E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using RPP30 Recombinant antibody (83481-5-RR)

    IF Staining of U-251 using 83481-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (83481-5-RR, Clone: 240468E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U-251 cells using RPP30 Recombinant antibody (83481-5-RR)

    FC experiment of U-251 using 83481-5-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug RPP30 Recombinant antibody (83481-5-RR, Clone:240468E4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83481-5-RR

    Affinity and Kinetic Characterization of 83481-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83481-5-RR against Human RPP30 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

RPP30 Recombinant monoclonal antibody

RPP30 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83481-5-RR
Clone No.240468E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

240468E4, RNase P subunit 2, RNaseP protein p30, RNASEP2, TSG15

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, U-251 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83481-5-RR targets RPP30 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8600

Product name: Recombinant human RPP30 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-268 aa of BC006991

Sequence: MAVFADLDLRAGSDLKALRGLVETAAHLGYSVVAINHIVDFKEKKQEIEKPVAVSELFTTLPIVQGKSRPIKILTRLTIIVSDPSHCNVLRATSSRARLYDVVAVFPKTEKLFHIACTHLDVDLVCITVTEKLPFYFKRPPINVAIDRGLAFELVYSPAIKDSTMRRYTISSALNLMQICKGKNVIISSAAERPLEIRGPYDVANLGLLFGLSESDAKAAVSTNCRAALLHGETRKTAFGIISTVKKPRPSEGDEDCLPASKKAKCEG

相同性解析による交差性が予測される生物種
Full Name ribonuclease P/MRP 30kDa subunit
Calculated molecular weight 268 aa, 29 kDa
Observed molecular weight30 kDa
GenBank accession numberBC006991
Gene Symbol RPP30
Gene ID (NCBI) 10556
RRIDAB_3671109
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP78346
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPP30 is a part of multimeric ribonuclease P complex and ribonuclease MRP complex. It is also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.

Protocols

Product Specific Protocols
IF protocol for RPP30 antibody 83481-5-RRDownload protocol
IHC protocol for RPP30 antibody 83481-5-RRDownload protocol
WB protocol for RPP30 antibody 83481-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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