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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using BIM/BCL2L11 Recombinant monoclonal antibody (81976-3-RR)

    WB analysis of PC-12 using 81976-3-RR (same clone as 81976-3-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 81976-3-RR (BIM, BCL2L11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81976-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using BIM/BCL2L11 Recombinant monoclonal antibody (81976-3-RR)

    WB analysis of mouse thymus using 81976-3-RR (same clone as 81976-3-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 81976-3-RR (BIM, BCL2L11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81976-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A20 cells using BIM/BCL2L11 Recombinant monoclonal antibody (81976-3-RR)

    WB analysis of A20 using 81976-3-RR (same clone as 81976-3-PBS)

    A20 cells were subjected to SDS PAGE followed by western blot with 81976-3-RR (BIM, BCL2L11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81976-3-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02863-1

    Cytometric bead array sample test of MP02863-1

    The mean BIM/BCL2L11 concentration was determined to be 4.4 ng/mL in Raji cell extract based on a 1.3 mg/mL extract load, 1.7 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02863-1

    Cytometric bead array standard curve of MP02863-1

    Cytometric bead array standard curve of MP02863-1, BIM/BCL2L11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81976-3-PBS. Detection antibody: 81976-2-PBS. Standard: Ag11875. Range: 0.156-20 ng/mL.

  • Affinity of 81976-3-RR

    Affinity of 81976-3-RR

    Biolayer interferometry (BLl) kinetic assays of 81976-3-RR against Human BIM/BCL2L11 were performed. The affinity constant is below 1 pM.

BIM/BCL2L11 Recombinant monoclonal antibody, PBS Only (Capture)

BIM/BCL2L11 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 81976-3-PBS
Clone No.252250A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

BCL2L11, BAM, Bcl2-interacting mediator of cell death, Bcl2-L-11, BCL2L11, BIM

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81976-3-PBS targets BIM/BCL2L11 as part of a matched antibody pair:

MP02863-1: 81976-3-PBS capture and 81976-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11875

Product name: Recombinant human BIM, BCL2L11 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-198 aa of BC033694

Sequence: MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQGNPEGNHGGEGDSCPHGSPQGPLAPPASPGPFATRSPLFIFMRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASMRQAEPADMRPEIWIAQELRRIGDEFNAYYARRVFLNNYQAAEDHPRMVILRLLRYIVRLVWRMH

相同性解析による交差性が予測される生物種
Full Name BCL2-like 11 (apoptosis facilitator)
Calculated molecular weight 198 aa, 22 kDa
Observed molecular weight16, 23 kDa
GenBank accession numberBC033694
Gene Symbol Bim
Gene ID (NCBI) 10018
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO43521
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BIM (BCL2L11) is a central BH3-only apoptosis activator that antagonizes anti-apoptotic BCL-2 proteins and directly triggers BAX/BAK-mediated mitochondrial permeabilization. Induced by cellular stress and cytokine withdrawal, BIM governs developmental cell death, immune tolerance, and tumor suppression. Alterations in BIM expression or splicing-particularly the deletion polymorphism affecting its BH3-only activity-underlie cancer therapy resistance, autoimmune disorders, and neuronal apoptosis.

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