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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using BIM/BCL2L11 Recombinant monoclonal antibody (81976-3-RR)

    WB analysis of PC-12 using 81976-3-RR

    PC-12 cells were subjected to SDS PAGE followed by western blot with 81976-3-RR (BIM, BCL2L11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using BIM/BCL2L11 Recombinant monoclonal antibody (81976-3-RR)

    WB analysis of mouse thymus using 81976-3-RR

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 81976-3-RR (BIM, BCL2L11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A20 cells using BIM/BCL2L11 Recombinant monoclonal antibody (81976-3-RR)

    WB analysis of A20 using 81976-3-RR

    A20 cells were subjected to SDS PAGE followed by western blot with 81976-3-RR (BIM, BCL2L11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity of 81976-3-RR

    Affinity of 81976-3-RR

    Biolayer interferometry (BLl) kinetic assays of 81976-3-RR against Human BIM/BCL2L11 were performed. The affinity constant is below 1 pM.

BIM/BCL2L11 Recombinant monoclonal antibody

BIM/BCL2L11 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 81976-3-RR
Clone No.252250A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

BCL2L11, BAM, Bcl2-interacting mediator of cell death, Bcl2-L-11, BCL2L11, BIM

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-12 cells, A20 cells, mouse thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81976-3-RR targets BIM/BCL2L11 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11875

Product name: Recombinant human BIM, BCL2L11 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-198 aa of BC033694

Sequence: MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQGNPEGNHGGEGDSCPHGSPQGPLAPPASPGPFATRSPLFIFMRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASMRQAEPADMRPEIWIAQELRRIGDEFNAYYARRVFLNNYQAAEDHPRMVILRLLRYIVRLVWRMH

相同性解析による交差性が予測される生物種
Full Name BCL2-like 11 (apoptosis facilitator)
Calculated molecular weight 198 aa, 22 kDa
Observed molecular weight16, 23 kDa
GenBank accession numberBC033694
Gene Symbol Bim
Gene ID (NCBI) 10018
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO43521
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BIM (BCL2L11) is a central BH3-only apoptosis activator that antagonizes anti-apoptotic BCL-2 proteins and directly triggers BAX/BAK-mediated mitochondrial permeabilization. Induced by cellular stress and cytokine withdrawal, BIM governs developmental cell death, immune tolerance, and tumor suppression. Alterations in BIM expression or splicing-particularly the deletion polymorphism affecting its BH3-only activity-underlie cancer therapy resistance, autoimmune disorders, and neuronal apoptosis.

Protocols

Product Specific Protocols
WB protocol for BIM/BCL2L11 antibody 81976-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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