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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-P53 (Ser46) Recombinant monoclonal antibod (80077-9-RR)

    WB analysis using 80077-9-RR

    Non-treated HT-29 cells and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80077-9-RR (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of A431 cells using Phospho-P53 (Ser46) Recombinant monoclonal antibod (80077-9-RR)

    WB analysis of A431 using 80077-9-RR

    Non-treated A431 cells and nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 80077-9-RR (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using Phospho-P53 (Ser46) Recombinant monoclonal antibod (80077-9-RR)

    IF Staining of HT-29 using 80077-9-RR

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser46) antibody (80077-9-RR, Clone: 250307A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-P53 (Ser46) Recombinant monoclonal antibody

Phospho-P53 (Ser46) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80077-9-RR
Clone No.250307A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Phospho P53, Phospho P53 (Ser46), Phospho-P53, Phospho-P53(Ser46), Phospho-P53-Ser46

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inetoposide treated HT-29 cells, nocodazole treated A431 cells
Positive IF/ICC detected inetoposide treated HT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80077-9-RR targets Phospho-P53 (Ser46) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight53 kDa
GenBank accession numberBC003596
Gene Symbol P53
Gene ID (NCBI) 7157
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP04637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Homeodomain-interacting protein kinase 2 (HIPK2) Phosphorylates Ser46 off p53 and p38 can phosphorylate this site.

Protocols

Product Specific Protocols
IF protocol for Phospho-P53 (Ser46) antibody 80077-9-RRDownload protocol
WB protocol for Phospho-P53 (Ser46) antibody 80077-9-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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