Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, 4T1 cells, HEK-293 cells, LNCaP cells, HeLa cells, HEK-293T cells, Jurkat cells, Raji cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68468-1-Ig targets PPA2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10125 Product name: Recombinant human PPA2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-305 aa of BC057219 Sequence: MSALLRLLRTGAPAAACLRLGTSAGTGSRRAMALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKVNSKEENGIPMKKARNDEYENLFNMIVEIPRWTNAKMEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYIWNYGTLPQILSCGEVIHVKILGILALIDEGETDWKLIAINANDPEASKFHDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCTNVQISDSPFRCTQEEARSLVESVSSSPNKESNEEEQVWHFLGK 相同性解析による交差性が予測される生物種 |
| Full Name | pyrophosphatase (inorganic) 2 |
| Calculated molecular weight | 305aa,35 kDa; 334aa,38 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC057219 |
| Gene Symbol | PPA2 |
| Gene ID (NCBI) | 27068 |
| RRID | AB_3085179 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9H2U2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPA2 antibody 68468-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |