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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PPA2 Monoclonal antibody (68468-1-Ig)

    WB analysis of HEK-293 using 68468-1-Ig (same clone as 68468-1-PBS)

    WB result of PPA2 antibody (68468-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPA2 transfected HEK-293 cells. This data was developed using the same antibody clone with 68468-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPA2 Monoclonal antibody (68468-1-Ig)

    WB analysis using 68468-1-Ig (same clone as 68468-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68468-1-Ig (PPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68468-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using PPA2 Monoclonal antibody (68468-1-Ig)

    WB analysis of 4T1 using 68468-1-Ig (same clone as 68468-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 68468-1-Ig (PPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68468-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PPA2 Monoclonal antibody, PBS Only

PPA2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68468-1-PBS
Clone No.1C2E3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

1C2E3, EC:3.6.1.1, HSPC124, PPase 2, Pyrophosphatase SID6 306

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68468-1-PBS targets PPA2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag10125

Product name: Recombinant human PPA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-305 aa of BC057219

Sequence: MSALLRLLRTGAPAAACLRLGTSAGTGSRRAMALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKVNSKEENGIPMKKARNDEYENLFNMIVEIPRWTNAKMEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYIWNYGTLPQILSCGEVIHVKILGILALIDEGETDWKLIAINANDPEASKFHDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCTNVQISDSPFRCTQEEARSLVESVSSSPNKESNEEEQVWHFLGK

相同性解析による交差性が予測される生物種
Full Name pyrophosphatase (inorganic) 2
Calculated molecular weight305aa,35 kDa; 334aa,38 kDa
Observed molecular weight34 kDa
GenBank accession numberBC057219
Gene Symbol PPA2
Gene ID (NCBI) 27068
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9H2U2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).

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