Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HL-60 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
68374-1-Ig targets ESYT2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20440 Product name: Recombinant human FAM62B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 559-773 aa of BC013957 Sequence: NPVVQMSVGHKAQESKIRYKTNEPVWEENFTFFIHNPKRQDLEVEVRDEQHQCSLGNLKVPLSQLLTSEDMTVSQRFQLSNSGPNSTIKMKIALRVLHLEKRERPPDHQHSAQVKRPSVSKEGRKTSIKSHMSGSPGPGGSNTAPSTPVIGGSDKPGMEEKAQPPEAGPQGLHDLGRSSSSLLASPGHISVKEPTPSIASDISLPIATQELRQRL 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 62 (C2 domain containing) member B |
| Calculated molecular weight | 921 aa, 102 kDa |
| Observed molecular weight | 90-100 kDa |
| GenBank accession number | BC013957 |
| Gene Symbol | ESYT2 |
| Gene ID (NCBI) | 57488 |
| RRID | AB_3085093 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | A0FGR8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ESYT2, also named FAM62B, is one of the three-member family of Extended Synaptotagmins that were recently shown to be implicated in the formation of endoplasmic reticulum (ER)-plasma membrane (PM) junctions and in the Ca(2+) dependent regulation of these junctions.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ESYT2 antibody 68374-1-Ig | Download protocol |
| WB protocol for ESYT2 antibody 68374-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |