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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ESYT2 Monoclonal antibody (68374-1-Ig)

    WB analysis using 68374-1-Ig (same clone as 68374-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (FAM62B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ESYT2 Monoclonal antibody (68374-1-Ig)

    WB analysis using 68374-1-Ig (same clone as 68374-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (ESYT2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ESYT2 Monoclonal antibody (68374-1-Ig)

    WB analysis of HeLa using 68374-1-Ig (same clone as 68374-1-PBS)

    WB result of FAM62B antibody (68374-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM62B transfected HeLa cells. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ESYT2 Monoclonal antibody (68374-1-Ig)

    WB analysis using 68374-1-Ig (same clone as 68374-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68374-1-Ig (FAM62B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ESYT2 Monoclonal antibody (68374-1-Ig)

    IF Staining of MCF-7 using 68374-1-Ig (same clone as 68374-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ESYT2 antibody (68374-1-Ig, Clone: 1B12G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ESYT2 Monoclonal antibody, PBS Only

ESYT2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68374-1-PBS
Clone No.1B12G2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FAM62B, Chr2Syt, E Syt2, E-Syt2, Extended synaptotagmin 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68374-1-PBS targets ESYT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag20440

Product name: Recombinant human FAM62B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 559-773 aa of BC013957

Sequence: NPVVQMSVGHKAQESKIRYKTNEPVWEENFTFFIHNPKRQDLEVEVRDEQHQCSLGNLKVPLSQLLTSEDMTVSQRFQLSNSGPNSTIKMKIALRVLHLEKRERPPDHQHSAQVKRPSVSKEGRKTSIKSHMSGSPGPGGSNTAPSTPVIGGSDKPGMEEKAQPPEAGPQGLHDLGRSSSSLLASPGHISVKEPTPSIASDISLPIATQELRQRL

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 62 (C2 domain containing) member B
Calculated molecular weight 921 aa, 102 kDa
Observed molecular weight90-100 kDa
GenBank accession numberBC013957
Gene Symbol ESYT2
Gene ID (NCBI) 57488
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDA0FGR8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ESYT2, also named FAM62B, is one of the three-member family of Extended Synaptotagmins that were recently shown to be implicated in the formation of endoplasmic reticulum (ER)-plasma membrane (PM) junctions and in the Ca(2+) dependent regulation of these junctions.

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