Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of human blood using 66778-1-Ig
human blood were subjected to SDS PAGE followed by western blot with 66778-1-Ig (GYPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 66778-1-Ig
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 66778-1-Ig
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human spleen using 66778-1-Ig
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human spleen using 66778-1-Ig
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 66778-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 66778-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human placenta using 66778-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human placenta using 66778-1-Ig
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Glycophorin A/CD235a Monoclonal antibody

Glycophorin A/CD235a Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 66778-1-Ig

Clone No.2B12G10

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

Glycophorin A, 2B12G10, CD235a, Glycophorin-A, GPA

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human blood tissue
Positive IHC detected in	human placenta tissue, human stomach cancer tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human placenta tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:6000
Immunohistochemistry (IHC)	IHC : 1:10000-1:40000
Immunofluorescence (IF)-P	IF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

66778-1-Ig targets Glycophorin A/CD235a in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag8635 Product name: Recombinant human GYPA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-150 aa of BC005319 Sequence: EVAMHTSTSSSVTKSYISSQTNDTHKRDTYAATPRAHEVSEISVRTVYPPEEETGERVQLAHHFSEPEITLIIFGVMAGVIGTILLISYGIRRLIKKSPSDVKPLPSPDTDVPLSSVEIENPETSDQ 相同性解析による交差性が予測される生物種
Full Name	glycophorin A (MNS blood group)
Calculated molecular weight	150 aa, 16 kDa
Observed molecular weight	36-38 kDa
GenBank accession number	BC005319
Gene Symbol	Glycophorin A
Gene ID (NCBI)	2993
RRID	AB_2882124
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P02724
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

Protocols

Product Specific Protocols
WB protocol for Glycophorin A/CD235a antibody 66778-1-Ig	Download protocol
IHC protocol for Glycophorin A/CD235a antibody 66778-1-Ig	Download protocol
IF protocol for Glycophorin A/CD235a antibody 66778-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Biochem C-terminally truncation is a prominent post-translational modification of human erythrocyte α-synuclein Authors - Ryosuke Amagai View Article

Target

Host

Species Reactivity