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Validation Data Gallery

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  • Western Blot (WB) analysis of human blood using Glycophorin A/CD235a Monoclonal antibody (66778-1-Ig)

    WB analysis of human blood using 66778-1-Ig (same clone as 66778-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66778-1-Ig (GYPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Glycophorin A/CD235a Monoclonal antibody (66778-1-Ig)

    IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Glycophorin A/CD235a Monoclonal antibody (66778-1-Ig)

    IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using Glycophorin A/CD235a Monoclonal antibody (66778-1-Ig)

    IHC staining of human spleen using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using Glycophorin A/CD235a Monoclonal antibody (66778-1-Ig)

    IHC staining of human spleen using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Glycophorin A/CD235a Monoclonal antibody (66778-1-Ig)

    IHC staining of human stomach cancer using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Glycophorin A/CD235a Monoclonal antibody (66778-1-Ig)

    IHC staining of human stomach cancer using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Glycophorin A/CD235a Monoclonal antibody (66778-1-Ig)

    IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Glycophorin A/CD235a Monoclonal antibody (66778-1-Ig)

    IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50422-2

    Cytometric bead array standard curve of MP50422-2

    Cytometric bead array standard curve of MP50422-2, GYPA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66778-2-PBS. Detection antibody: 66778-1-PBS. Standard:Ag8635. Range: 0.391-100 ng/mL.

Glycophorin A/CD235a Monoclonal antibody, PBS Only (Detector)

Glycophorin A/CD235a Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 66778-1-PBS
Clone No.2B12G10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

Glycophorin A, 2B12G10, CD235a, Glycophorin-A, GPA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66778-1-PBS targets Glycophorin A/CD235a as part of a matched antibody pair:

MP50422-2: 66778-2-PBS capture and 66778-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8635

Product name: Recombinant human GYPA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-150 aa of BC005319

Sequence: EVAMHTSTSSSVTKSYISSQTNDTHKRDTYAATPRAHEVSEISVRTVYPPEEETGERVQLAHHFSEPEITLIIFGVMAGVIGTILLISYGIRRLIKKSPSDVKPLPSPDTDVPLSSVEIENPETSDQ

相同性解析による交差性が予測される生物種
Full Name glycophorin A (MNS blood group)
Calculated molecular weight 150 aa, 16 kDa
Observed molecular weight 36-38 kDa
GenBank accession numberBC005319
Gene Symbol Glycophorin A
Gene ID (NCBI) 2993
RRIDAB_2882124
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP02724
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)