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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of human placenta using 66112-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of pig brain using 66112-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of rat brain using 66112-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of mouse brain using 66112-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of human brain using 66112-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of human placenta using 66112-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    WB analysis of human brain using 66112-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    IHC staining of human liver using 66112-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    IHC staining of human liver using 66112-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Iduronate 2 sulfatase Monoclonal antibody (66112-1-Ig)

    IF Staining of HepG2 using 66112-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Iduronate 2 sulfatase antibody (66112-1-Ig, Clone: 1A3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

Iduronate 2 sulfatase Monoclonal antibody

Iduronate 2 sulfatase Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66112-1-Ig
Clone No.1A3F9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IDS, 1A3F9, Alpha-L-iduronate sulfate sulfatase, EC:3.1.6.13, Iduronate 2-sulfatase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHuman placenta, rat brain tissue, human brain tissue, mouse brain tissue, pig brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66112-1-Ig targets Iduronate 2 sulfatase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19095

Product name: Recombinant human IDS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-312 aa of BC006170

Sequence: MPPPRTGRGLLWLGLVLSSVCVALGSETQANSTTDALNVLLIIVDDLRPSLGCYGDKLVRSPNIDQLASHSLLFQNAFAQQAVCAPSRVSFLTGRRPDTTRLYDFNSYWRVHAGNFSTIPQYFKENGYVTMSVGKVFHPGISSNHTDDSPYSWSFPPYHPSSEKYENTKTCRGPDGELHANLLCPVDVLDVPEGTLPDKQSTEQAIQLLEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPEVPDGLPPVAYNPWMDIRQREDVQALNISVPYGPIPVDFQEDQSSTGFRLKTSSTRKYK

相同性解析による交差性が予測される生物種
Full Name iduronate 2-sulfatase
Calculated molecular weight312aa,35 kDa; 550aa,62 kDa
Observed molecular weight 76 kDa, 55 kDa
GenBank accession numberBC006170
Gene Symbol IDS
Gene ID (NCBI) 3423
RRIDAB_2881511
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP22304
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).

Protocols

Product Specific Protocols
WB protocol for Iduronate 2 sulfatase antibody 66112-1-IgDownload protocol
IHC protocol for Iduronate 2 sulfatase antibody 66112-1-IgDownload protocol
IF protocol for Iduronate 2 sulfatase antibody 66112-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols