Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICCFC (Intra)Cytometric bead arrayAffinity

WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)
WB result of IL-18 antibody (60070-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-18 transfected HeLa cells. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)
WB result of Pre-IL18 antibody (60070-1-Ig, 1:4000) with si-Control and si-Pre-IL18 transfected HeLa cells.. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 60070-1-Ig (same clone as 60070-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 60070-1-Ig (same clone as 60070-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-18 antibody (60070-1-Ig, Clone: 5C6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (60070-1-Ig, Clone: 5C6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.4 ug IL-18 Monoclonal antibody (60070-1-Ig, Clone:5C6F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
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1x10^6 HeLa cells were intracellularly stained with 0.4 ug IL-18 Monoclonal antibody (60070-1-Ig, Clone:5C6F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP51140-3
Cytometric bead array standard curve of MP51140-3, IL-18 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60070-1-PBS. Detection antibody: 60070-2-PBS. Standard:Ag1063. Range: 0.098-100 ng/mL.
Affinity and Kinetic Characterization of 60070-1-Ig
Biolayer interferometry (BLl) kinetic assays of 60070-1-Ig against Human Pre-IL18 were performed. The Affinity Constant is 2.79 nM.

Featured Product
KD/KO Validated

IL-18 Monoclonal antibody, PBS Only (Capture)

IL-18 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 60070-1-PBS

Clone No.5C6F8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

IL18, Pre-IL18, 5C6F8, Iboctadekin, IFN-gamma-inducing factor

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

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MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60070-1-PBS targets IL-18 as part of a matched antibody pair:

MP51140-3: 60070-1-PBS capture and 60070-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag1063 Product name: Recombinant human Pre IL-18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC007461 Sequence: MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED 相同性解析による交差性が予測される生物種
Full Name	IL-18
Calculated molecular weight	193 aa, 22 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC007461
Gene Symbol	IL-18
Gene ID (NCBI)	3606
RRID	AB_2280158
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q14116
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

IL-18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)

WB analysis of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

WB analysis of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)

IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

IHC staining of human stomach cancer using 60070-1-Ig (same clone as 60070-1-PBS)

IHC staining of human stomach cancer using 60070-1-Ig (same clone as 60070-1-PBS)

IF Staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

IF Staining of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

FC experiment of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

Cytometric bead array standard curve of MP51140-3

Affinity and Kinetic Characterization of 60070-1-Ig