Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells, A549 cells, HepG2 cells |
| Positive IHC detected in | human tonsillitis tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 48 publications below |
| IHC | See 7 publications below |
| IF | See 10 publications below |
| ELISA | See 1 publications below |
Product Information
60070-1-Ig targets IL-18 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1063 Product name: Recombinant human Pre IL-18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC007461 Sequence: MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED 相同性解析による交差性が予測される生物種 |
| Full Name | IL-18 |
| Calculated molecular weight | 193 aa, 22 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC007461 |
| Gene Symbol | IL-18 |
| Gene ID (NCBI) | 3606 |
| RRID | AB_2280158 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL-18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IL-18 antibody 60070-1-Ig | Download protocol |
| IF protocol for IL-18 antibody 60070-1-Ig | Download protocol |
| IHC protocol for IL-18 antibody 60070-1-Ig | Download protocol |
| WB protocol for IL-18 antibody 60070-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Mast cells boost anti-tumor potency of MAIT cells via inflammasome-dependent secretion of IL-18 | ||
J Adv Res Mitochondrial dysfunction drives ZBP1-mediated PANoptosis to increase the susceptibility of heart failure with preserved ejection fraction-associated atrial fibrillation. | ||
Cell Death Dis PACS-2 deficiency aggravates tubular injury in diabetic kidney disease by inhibiting ER-phagy | ||
Int J Biol Sci Cisplatin Induces Pyroptosis via Activation of MEG3/NLRP3/caspase-1/GSDMD Pathway in Triple-Negative Breast Cancer. | ||
J Neuroinflammation GPR120 modulates epileptic seizure and neuroinflammation mediated by NLRP3 inflammasome. |