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HINT1 Polyclonal antibody

HINT1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10717-1-AP

Synonyms

HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1



Tested Applications

Positive WB detected inJurkat cells, rat brain tissue, rat thymus tissue, K-562 cells, Raji cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10717-1-AP targets HINT1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HINT1 fusion protein Ag1142
Full Name histidine triad nucleotide binding protein 1
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC007090
Gene Symbol HINT1
Gene ID (NCBI) 3094
RRIDAB_2248272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.This antibody is specific to HINT1.

Protocols

Product Specific Protocols
WB protocol for HINT1 antibody 10717-1-APDownload protocol
IHC protocol for HINT1 antibody 10717-1-APDownload protocol
IP protocol for HINT1 antibody 10717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells.

Authors - Jing Yu

Oncogene

Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells.

Authors - A Motzik
humanWB

J Med Chem

A Chemical Strategy for Intracellular Arming of an Endogenous Broad-Spectrum Antiviral Nucleotide

Authors - Kellan T. Passow
humanIHC

Cell Cycle

The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells.

Authors - Genovese Giannicola G
human,mouseWB

Pharmacogenomics J

Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence.

Authors - Jackson K J KJ
mouseWB,IHC

J Proteome Res

Novel breast cancer metastasis-associated proteins.

Authors - Ho Jiapei J