Validation Data Gallery

Filter: AllWBIPIHCFC (Intra)

WB analysis of HeLa using 12273-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 12273-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12273-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12273-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12273-1-AP
IP result of anti-SSSCA1 (IP:12273-1-AP, 4ug; Detection:12273-1-AP 1:1000) with HeLa cells lysate 2000ug.
IHC staining of mouse testis using 12273-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 12273-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HeLa using 12273-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug SSSCA1 Polyclonal antibody (12273-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

SSSCA1 Polyclonal antibody

SSSCA1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 12273-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Synonyms

ZNRD2, Autoantigen p27, p27, Protein ZNRD2, Zinc ribbon domain-containing protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, NIH/3T3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below

Product Information

12273-1-AP targets SSSCA1 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2916 Product name: Recombinant human SSSCA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-199 aa of BC014791 Sequence: MALNGAEVDDFSWEPPTEAETKVLQARRERQDRISRLMGDYLLRGYRMLGETCADCGTILLQDKQRKIYCVACQELDSDVDKDNPALNAQAALSQAREHQLASASELPLGSRPAPQPPVPRPEHCEGAAAGLKAAQGPPAPAVPPNTDVMACTQTALLQKLTWASAELGSSTSLETSIQLCGLIRACAEALRSLQQLQH 相同性解析による交差性が予測される生物種
Full Name	Sjogren syndrome/scleroderma autoantigen 1
Calculated molecular weight	199 aa, 21 kDa
Observed molecular weight	27 kDa
GenBank accession number	BC014791
Gene Symbol	SSSCA1
Gene ID (NCBI)	10534
RRID	AB_2195902
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60232
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about23- 27 kDa.

Protocols

Product Specific Protocols
IHC protocol for SSSCA1 antibody 12273-1-AP	Download protocol
IP protocol for SSSCA1 antibody 12273-1-AP	Download protocol
WB protocol for SSSCA1 antibody 12273-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Mechanism of orphan subunit recognition during assembly quality control Authors - Yuichi Yagita KO Validated View Article
mouse,human	WB,IHC	J Ethnopharmacol Centipeda minima extracts and the active sesquiterpene lactones have therapeutic efficacy in non-small cell lung cancer by suppressing Skp2/p27 signaling pathway Authors - Han-Chen Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 12273-1-AP

WB analysis of NIH/3T3 using 12273-1-AP

WB analysis of A549 using 12273-1-AP