• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated transgelin/SM22 Monoclonal antibody

transgelin/SM22 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A10C2

Cat no : CL594-60213

Synonyms

22 kDa actin binding protein, DKFZp686P11128, Protein WS3 10, SM22, SM22 alpha, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1, transgelin/SM22, WS3 10

Validation Data Gallery

Applications:
  • IF Staining of mouse heart using CL594-60213

    IF Staining of mouse heart using CL594-60213

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL594-60213 (transgelin/SM22 antibody) at dilution of 1:100.

  • IF Staining of mouse heart using CL594-60213

    IF Staining of mouse heart using CL594-60213

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL594-60213 (transgelin/SM22 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy TAGLN Ready-To-Use IHC Kit. TAGLN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60213-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-60213 is transgelin/SM22 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764 相同性解析による交差性が予測される生物種
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 18+22 kDa
GenBank accession numberBC004927
Gene symbol TAGLN
Gene ID (NCBI) 6876
RRIDAB_2883444
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.

Protocols

Product Specific Protocols
IF protocol for CL594 transgelin/SM22 antibody CL594-60213Download protocol
Standard Protocols
Click here to view our Standard Protocols