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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated pan-keratin Polyclonal antibody

pan-keratin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-26411

Synonyms

58 kDa cytokeratin, CK 5, CK5, Cytokeratin 5, K5, keratin 5, KRT5, KRT5A, pan-keratin, Type II keratin Kb5

Validation Data Gallery

Applications:
  • IF Staining of human colon cancer using CL555-26411

    IF Staining of human colon cancer using CL555-26411

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

  • IF Staining of human colon cancer using CL555-26411

    IF Staining of human colon cancer using CL555-26411

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

  • IF Staining of human lung cancer using CL555-26411

    IF Staining of human lung cancer using CL555-26411

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200, CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3, Magenta).

  • IF Staining of human lung cancer using CL555-26411

    IF Staining of human lung cancer using CL555-26411

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200, CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3, Magenta).

  • IF Staining of A431 using CL555-26411

    IF Staining of A431 using CL555-26411

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

  • IF Staining of A431 using CL555-26411

    IF Staining of A431 using CL555-26411

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

  • IF Staining of human lung cancer using CL555-26411

    IF Staining of human lung cancer using CL555-26411

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

  • IF Staining of human lung cancer using CL555-26411

    IF Staining of human lung cancer using CL555-26411

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

  • IF Staining of human breast cancer using CL555-26411

    IF Staining of human breast cancer using CL555-26411

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

  • IF Staining of human breast cancer using CL555-26411

    IF Staining of human breast cancer using CL555-26411

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman colon cancer tissue, A431 cells, human breast cancer tissue, human lung cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 26411-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL555-26411 targets pan-keratin in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen pan-keratin fusion protein Ag24184 相同性解析による交差性が予測される生物種
Full Name keratin 5
Calculated molecular weight 590 aa, 62 kDa
GenBank accession numberBC024292
Gene symbol KRT5
Gene ID (NCBI) 3852
RRIDAB_2919645
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.

Protocols

Product Specific Protocols
IF protocol for CL555 pan-keratin antibody CL555-26411Download protocol
Standard Protocols
Click here to view our Standard Protocols