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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated eIF4G2/DAP5 Monoclonal antibody

eIF4G2/DAP5 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3C11A3

Cat no : CL555-67428

Synonyms

AAG1, DAP 5, DAP5, Death associated protein 5, eIF 4 gamma 2, eIF 4G 2, eIF4G 2, eIF4G2, FLJ41344, NAT1, p97

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL555-67428

    IF Staining of HepG2 using CL555-67428

    Immunofluorescent analysis of (-20°C Ethanol) fixed G2' target='_blank'>HepG2 cells using CoraLite®555 eIF4G2/DAP5 antibody (CL555-67428, Clone: 3C11A3 ) at dilution of 1:600.

  • FC experiment of HeLa using CL555-67428

    FC experiment of HeLa using CL555-67428

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human eIF4G2/DAP5 (CL555-67428, Clone:3C11A3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EIF4G2 Ready-To-Use IHC Kit. EIF4G2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67428-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:300-1:1200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL555-67428 targets eIF4G2/DAP5 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen eIF4G2/DAP5 fusion protein Ag12080 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4 gamma, 2
Calculated molecular weight 907 aa, 102 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC043149
Gene symbol EIF4G2
Gene ID (NCBI) 1982
RRIDAB_2919702
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL555 eIF4G2/DAP5 antibody CL555-67428Download protocol
FC protocol for CL555 eIF4G2/DAP5 antibody CL555-67428Download protocol
Standard Protocols
Click here to view our Standard Protocols