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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

calreticulin Recombinant antibody

calreticulin Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B17

Cat no : 80017-1-RR

Synonyms

CALR, Calregulin, calreticulin, cC1qR, CRP55, CRT, CRTC, ERp60, FLJ26680, grp60, HACBP, RO, SSA

Validation Data Gallery

Applications:
  • WB analysis using 80017-1-RR

    WB analysis using 80017-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80017-1-RR (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 80017-1-RR

    IHC staining of human thyroid cancer using 80017-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 80017-1-RR

    IHC staining of human thyroid cancer using 80017-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 80017-1-RR

    IHC staining of human breast cancer using 80017-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 80017-1-RR

    IHC staining of human breast cancer using 80017-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman thyroid cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

80017-1-RR targets calreticulin in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen calreticulin fusion protein Ag26064 相同性解析による交差性が予測される生物種
Full Name calreticulin
Calculated molecular weight 60 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC002500
Gene symbol CALR
Gene ID (NCBI) 811
RRIDAB_2882941
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for calreticulin antibody 80017-1-RRDownload protocol
IHC protocol for calreticulin antibody 80017-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols