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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated band 3/ AE1 Monoclonal antibody

band 3/ AE1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E5C1

Cat no : CL594-66984

Synonyms

AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, Band 3 anion transport protein, BND3, CD233, DI, EMPB3, EPB3, FR, RTA1A, SLC4A1, SW, WD, WD1, WR

Validation Data Gallery

Applications:
  • IF Staining of human placenta using CL594-66984

    IF Staining of human placenta using CL594-66984

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 band 3/ AE1 antibody (CL594-66984, Clone: 1E5C1 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Band 3/AE1 Ready-To-Use IHC Kit. Band 3/AE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66984-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66984 targets band 3/ AE1 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen band 3/ AE1 fusion protein Ag28058 相同性解析による交差性が予測される生物種
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Calculated molecular weight 911 aa, 102 kDa
GenBank accession numberBC101570
Gene symbol SLC4A1
Gene ID (NCBI) 6521
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)

Protocols

Product Specific Protocols
IF protocol for CL594 band 3/ AE1 antibody CL594-66984Download protocol
Standard Protocols
Click here to view our Standard Protocols