band 3/ AE1 Monoclonal antibody
band 3/ AE1 Monoclonal Antibody for IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1E5C1
Cat no : 66984-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human placenta tissue |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
66984-1-Ig targets band 3/ AE1 in IHC, IF, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | band 3/ AE1 fusion protein Ag28058 相同性解析による交差性が予測される生物種 |
Full Name | solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group) |
Calculated molecular weight | 911 aa, 102 kDa |
GenBank accession number | BC101570 |
Gene symbol | SLC4A1 |
Gene ID (NCBI) | 6521 |
RRID | AB_2918479 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)
Protocols
Product Specific Protocols | |
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IHC protocol for band 3/ AE1 antibody 66984-1-Ig | Download protocol |
IF protocol for band 3/ AE1 antibody 66984-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |