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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha SNAP Monoclonal antibody

Alpha SNAP Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E2A2

Cat no : 67323-1-Ig

Synonyms

Alpha SNAP, NAPA, SNAP alpha, SNAPA

Validation Data Gallery

Applications:
  • WB analysis using 67323-1-Ig

    WB analysis using 67323-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67323-1-Ig

    WB analysis using 67323-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 67323-1-Ig

    IHC staining of mouse cerebellum using 67323-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 67323-1-Ig

    IHC staining of mouse cerebellum using 67323-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 67323-1-Ig

    IF Staining of mouse brain using 67323-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 67323-1-Ig

    IF Staining of mouse brain using 67323-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67323-1-Ig targets Alpha SNAP in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha SNAP fusion protein Ag13180 相同性解析による交差性が予測される生物種
Full Name N-ethylmaleimide-sensitive factor attachment protein, alpha
Calculated molecular weight 33 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC007432
Gene symbol NAPA
Gene ID (NCBI) 8775
RRIDAB_2882583
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Alpha SNAP antibody 67323-1-IgDownload protocol
IHC protocol for Alpha SNAP antibody 67323-1-IgDownload protocol
IF protocol for Alpha SNAP antibody 67323-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols