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  • KD/KO Validated

ZFYVE16 Polyclonal antibody

ZFYVE16 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13118-2-AP

Synonyms

ENDOFIN, KIAA0305, ZFYVE16



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human placenta tissue, mouse kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human heart tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13118-2-AP targets ZFYVE16 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFYVE16 fusion protein Ag3811 相同性解析による交差性が予測される生物種
Full Name zinc finger, FYVE domain containing 16
Calculated molecular weight 1539 aa, 169 kDa
Observed molecular weight230-250 kDa
GenBank accession numberBC032227
Gene symbol ZFYVE16
Gene ID (NCBI) 9765
RRIDAB_2288795
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endofin (also known as ZFYVE16) is an endosomal FYVE domain protein implicated in regulating membrane trafficking. Endofin has been shown to localize to early endosomes. It co-localizes with SARA but does not associate with it or Smad2 and does not behave like SARA in affecting TGF-signaling. Northern blot analysis showed ZFYVE16 was widely expressed with high levels in kidney, placenta and lung. Overexpression of Endofin causes endosome aggregation. The gene of ZFYVE16 maps to chromosome 5q14, and encodes a 1539-amino acid protein with a molecular mass of 169 kDa. In addition, endogenous Endofin can also be detected as a band of 230-250 kDa. (PMID: 11546807)

Protocols

Product Specific Protocols
WB protocol for ZFYVE16 antibody 13118-2-APDownload protocol
IHC protocol for ZFYVE16 antibody 13118-2-APDownload protocol
IF protocol for ZFYVE16 antibody 13118-2-APDownload protocol
IP protocol for ZFYVE16 antibody 13118-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishIF

PLoS Genet

The Lowe Syndrome Protein OCRL1 Is Required for Endocytosis in the Zebrafish Pronephric Tubule.

Authors - Francesca Oltrabella
humanWB

Structure

Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.

Authors - Deepankar Gahloth
  • KD Validated
humanWB

iScience

Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.

Authors - Jalal M Kazan
  • KD Validated
humanWB

FEBS Lett

SARA is dispensable for functional TGF-β signaling.

Authors - Bakkeb? Maren M
  • KD Validated