• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZC3H12A Monoclonal antibody

ZC3H12A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F6B2

Cat no : 68616-1-Ig

Synonyms

dJ423B22.1, FLJ23231, MCP induced protein 1, MCPIP, MCPIP1, Ribonuclease ZC3H12A, RP3 423B22.1, ZC3H12A

Validation Data Gallery

Applications:
  • WB analysis using 68616-1-Ig

    WB analysis using 68616-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68616-1-Ig (ZC3H12A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Tested Applications

Positive WB detected inHT-1376 cells, BxPC-3 cells, MDA-MB-468 cells, Raji cells, THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy ZC3H12A Ready-To-Use IHC Kit. ZC3H12A primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68616-1-Ig targets ZC3H12A in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZC3H12A fusion protein Ag13877 相同性解析による交差性が予測される生物種
Full Name zinc finger CCCH-type containing 12A
Calculated molecular weight 599 aa, 66 kDa
Observed molecular weight66 kDa
GenBank accession numberBC005001
Gene symbol ZC3H12A
Gene ID (NCBI) 80149
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.

Protocols

Product Specific Protocols
WB protocol for ZC3H12A antibody 68616-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols