CRM1 Monoclonal antibody

CRM1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D3B6

Cat no : 66763-1-Ig

Synonyms

CRM1, DKFZp686B1823, Exp1, Exportin 1, XPO1



Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CRM1 Ready-To-Use IHC Kit. CRM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66763-1-Ig targets CRM1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRM1 fusion protein Ag27337 相同性解析による交差性が予測される生物種
Full Name exportin 1 (CRM1 homolog, yeast)
Calculated molecular weight 1071 aa, 123 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC032847
Gene symbol XPO1
Gene ID (NCBI) 7514
RRIDAB_2882109
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

Protocols

Product Specific Protocols
WB protocol for CRM1 antibody 66763-1-IgDownload protocol
IHC protocol for CRM1 antibody 66763-1-IgDownload protocol
IF protocol for CRM1 antibody 66763-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Diabetologia

Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Authors - Mu Zhang
WB

Sci Rep

Anti-tumor efficacy of Selinexor (KPT-330) in gastric cancer is dependent on nuclear accumulation of p53 tumor suppressor.

Authors - Vinod Vijay Subhash
mouseWB

Exp Gerontol

Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.

Authors - Shama R Iyer
humanWB

Exp Cell Res

NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.

Authors - Jianning Xu
humanIHC

PeerJ

Hub gene associated with prognosis in bladder cancer is a novel therapeutic target

Authors - Dengpan Fang
humanWB

Cell Death Dis

Synergistic antifibrotic effects of miR-451 with miR-185 partly by co-targeting EphB2 on hepatic stellate cells.

Authors - Xiaogang Chen