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- KD/KO Validated
NSD3 Polyclonal antibody
NSD3 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IHC, IF, FC, chIP, ELISA
Conjugate
Unconjugated
Cat no : 11345-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 6 publications below |
| IF | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
11345-1-AP targets NSD3 in WB, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NSD3 fusion protein Ag1894 相同性解析による交差性が予測される生物種 |
| Full Name | Wolf-Hirschhorn syndrome candidate 1-like 1 |
| Calculated molecular weight | 1437 aa, 162 kDa |
| Observed molecular weight | 160-170 kDa, 68-72 kDa |
| GenBank accession number | BC012059 |
| Gene symbol | WHSC1L1 |
| Gene ID (NCBI) | 54904 |
| RRID | AB_2216189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NSD3 antibody 11345-1-AP | Download protocol |
| IHC protocol for NSD3 antibody 11345-1-AP | Download protocol |
| IF protocol for NSD3 antibody 11345-1-AP | Download protocol |
| FC protocol for NSD3 antibody 11345-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res NSD3-Induced Methylation of H3K36 Activates NOTCH Signaling to Drive Breast Tumor Initiation and Metastatic Progression. | ||
Cancer Res Transforming properties of 8p11-12 amplified genes in human breast cancer.
| ||
Mol Oncol Amplification of WHSC1L1 regulates expression and estrogen-independent activation of ERα in SUM-44 breast cancer cells and is associated with ERα over-expression in breast cancer. | ||
Sci Rep NSD2 contributes to oncogenic RAS-driven transcription in lung cancer cells through long-range epigenetic activation. | ||
Sci Rep WHSC1L1-mediated EGFR mono-methylation enhances the cytoplasmic and nuclear oncogenic activity of EGFR in head and neck cancer.
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