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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Villin Polyclonal antibody

Villin Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16488-1-AP

Synonyms

VIL, VIL1, Villin, villin 1

Validation Data Gallery

Applications:
  • WB analysis of mouse kidney using 16488-1-AP

    WB analysis of mouse kidney using 16488-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16488-1-AP (Villin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 16488-1-AP

    WB analysis of mouse liver using 16488-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16488-1-AP (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse colon using 16488-1-AP

    WB analysis of mouse colon using 16488-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 16488-1-AP (Villin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 16488-1-AP

    IP experiment of mouse kidney using 16488-1-AP

    IP result of anti-Villin (IP:16488-1-AP, 4ug; Detection:16488-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

  • IHC staining of mouse small intestine using 16488-1-AP

    IHC staining of mouse small intestine using 16488-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16488-1-AP (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VIL1 antibody included
  • IHC staining of mouse small intestine using 16488-1-AP

    IHC staining of mouse small intestine using 16488-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16488-1-AP (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VIL1 antibody included
  • IHC staining of human colon cancer using 16488-1-AP

    IHC staining of human colon cancer using 16488-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16488-1-AP (Villin antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VIL1 antibody included
  • IHC staining of human colon cancer using 16488-1-AP

    IHC staining of human colon cancer using 16488-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16488-1-AP (Villin antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VIL1 antibody included
  • IF Staining of mouse small intestine using 16488-1-AP

    IF Staining of mouse small intestine using 16488-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 16488-1-AP

    IF Staining of mouse small intestine using 16488-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:200 and CoraLite®647-conjugated AffiniPure F(ab, CoraLite® Plus 488 PIGR antibody (CL488-22024, green), CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).

  • IF Staining of COLO 320 using 16488-1-AP

    IF Staining of COLO 320 using 16488-1-AP

    Immunofluorescent analysis of COLO 320 cells using 16488-1-AP (Villin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 16488-1-AP

    FC experiment of HepG2 using 16488-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Villin (16488-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse kidney tissue, mouse colon tissue, mouse liver tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse small intestine tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse small intestine tissue, COLO 320 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VIL1 Ready-To-Use IHC Kit. VIL1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

16488-1-AP targets Villin in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Villin fusion protein Ag9610 相同性解析による交差性が予測される生物種
Full Name villin 1
Calculated molecular weight827aa,93 kDa; 826aa,93 kDa
Observed molecular weight 93 kDa
GenBank accession numberBC017303
Gene symbol VIL1
Gene ID (NCBI) 7429
RRIDAB_2215975
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

Protocols

Product Specific Protocols
WB protocol for Villin antibody 16488-1-APDownload protocol
IHC protocol for Villin antibody 16488-1-APDownload protocol
IF protocol for Villin antibody 16488-1-APDownload protocol
IP protocol for Villin antibody 16488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Proc Natl Acad Sci U S A

Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface.

Authors - Jacqueline Corry
View Article
mouseIF

Clin Transl Med

NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions.

Authors - Zhixin Liu
View Article
pigIF

PLoS Pathog

Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids.

Authors - Qi-Yue Yang
View Article
mouseWB

Front Cell Dev Biol

Cathelicidin-WA Protects Against LPS-Induced Gut Damage Through Enhancing Survival and Function of Intestinal Stem Cells.

Authors - Sisi Wang
View Article
mouseIHC

Int J Mol Sci

Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays.

Authors - Wenyan Yang
View Article
mouseIHC

Toxicol In Vitro

Survival and cellular heterogeneity of epithelium in cultured mouse and rat precision-cut intestinal slices.

Authors - Carin Biel
View Article