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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated VASH2 Monoclonal antibody

VASH2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A11D10

Cat no : CL488-67753

Synonyms

FLJ12505, RP11 275G3.1, VASH2, VASHL, vasohibin 2, Vasohibin like protein

Validation Data Gallery

Applications:
  • IF Staining of SKOV-3 using CL488-67753

    IF Staining of SKOV-3 using CL488-67753

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 488 VASH2 antibody (CL488-67753, Clone: 3A11D10 ) at dilution of 1:200.

Tested Applications

Positive IF detected inSKOV-3 cells
For other applications, we recommend the unconjugated version of this antibody, 67753-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67753 targets VASH2 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VASH2 fusion protein Ag29985 相同性解析による交差性が予測される生物種
Full Name vasohibin 2
Calculated molecular weight 355 aa, 40 kDa
Observed molecular weight34 kDa
GenBank accession numberBC028194
Gene symbol VASH2
Gene ID (NCBI) 79805
RRIDAB_2919537
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. 67753-1-Ig antibody recognizes both the nuclear and cytoplasmic isoforms. (PMID:23615928; 19204325; 31235911; 26177649)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 VASH2 antibody CL488-67753Download protocol
Standard Protocols
Click here to view our Standard Protocols