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  • KD/KO Validated

USP15 Monoclonal antibody

USP15 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C9G3

Cat no : 67557-1-Ig

Synonyms

Deubiquitinating enzyme 15, KIAA0529, Ubiquitin thiolesterase 15, Unph 2, UNPH4, USP15



Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, 4T1 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67557-1-Ig targets USP15 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP15 fusion protein Ag5712 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 15
Calculated molecular weight 112 kDa
Observed molecular weight 112 kDa
GenBank accession numberBC020688
Gene symbol USP15
Gene ID (NCBI) 9958
RRIDAB_2882771
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for USP15 antibody 67557-1-IgDownload protocol
IHC protocol for USP15 antibody 67557-1-IgDownload protocol
IF protocol for USP15 antibody 67557-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Death Dis

The deubiquitinating enzyme USP15 stabilizes ERα and promotes breast cancer progression.

Authors - Xiaohong Xia
  • KD Validated
humanWB

Endocr Relat Cancer

LncRNA MFSD4A-AS1 promotes lymphangiogenesis and lymphatic metastasis of papillary thyroid cancer

Authors - Xiaoli Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shunbin (Verified Customer) (06-07-2023)

Great antibody for Western Blot. Very specific single band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5,000
USP15 Antibody Western Blot validation (1:5,000 dilution) in (Cat no:67557-1-Ig)