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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP15 Monoclonal antibody (67557-1-Ig)

    WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP15 Monoclonal antibody (67557-1-Ig)

    WB analysis of HeLa using 67557-1-Ig (same clone as 67557-1-PBS)

    WB result of USP15 antibody (67557-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP15 transfected HeLa cells. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP15 Monoclonal antibody (67557-1-Ig)

    WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using USP15 Monoclonal antibody (67557-1-Ig)

    WB analysis of 4T1 using 67557-1-Ig (same clone as 67557-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using USP15 Monoclonal antibody (67557-1-Ig)

    IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using USP15 Monoclonal antibody (67557-1-Ig)

    IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using USP15 Monoclonal antibody (67557-1-Ig)

    IF Staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using USP15 antibody (67557-1-Ig, Clone: 1C9G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

USP15 Monoclonal antibody, PBS Only

USP15 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67557-1-PBS
Clone No.1C9G3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

1C9G3, Deubiquitinating enzyme 15, EC:3.4.19.12, KIAA0529, Ubiquitin carboxyl-terminal hydrolase 15

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67557-1-PBS targets USP15 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5712

Product name: Recombinant human USP15 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-235 aa of BC020688

Sequence: MAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKETRLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPKKPLEQSC

相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 15
Calculated molecular weight 112 kDa
Observed molecular weight 112 kDa
GenBank accession numberBC020688
Gene Symbol USP15
Gene ID (NCBI) 9958
RRIDAB_2882771
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9Y4E8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.