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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UPF1 Recombinant antibody

UPF1 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3O15

Cat no : 82791-1-RR

Synonyms

ATP dependent helicase RENT1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1

Validation Data Gallery

Applications:
  • WB analysis using 82791-1-RR

    WB analysis using 82791-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82791-1-RR (UPF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82791-1-RR

    IF Staining of HepG2 using 82791-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UPF1 antibody (82791-1-RR, Clone: 3O15 ) at dilution of 1:370 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

Tested Applications

Positive WB detected inrat testis tissue, Raji cells, SH-SY5Y cells, HEK-293 cells, HepG2 cells, HeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:185-1:740
Sample-dependent, check data in validation data gallery

Product Information

82791-1-RR targets UPF1 in WB, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UPF1 fusion protein Ag29057 相同性解析による交差性が予測される生物種
Full Name UPF1 regulator of nonsense transcripts homolog (yeast)
Calculated molecular weight123 kDa
Observed molecular weight 123-130 kDa
GenBank accession numberBC039817
Gene symbol UPF1
Gene ID (NCBI) 5976
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

Protocols

Product Specific Protocols
WB protocol for UPF1 antibody 82791-1-RRDownload protocol
IF protocol for UPF1 antibody 82791-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols