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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Uromodulin Polyclonal antibody

Uromodulin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 29975-1-AP

Synonyms

ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, uromodulin

Validation Data Gallery

Applications:
  • WB analysis of human urine using 29975-1-AP

    WB analysis of human urine using 29975-1-AP

    human urine sample were subjected to SDS PAGE followed by western blot with 29975-1-AP (Uromodulin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 29975-1-AP

    IHC staining of human kidney using 29975-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29975-1-AP (Uromodulin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 29975-1-AP

    IHC staining of human kidney using 29975-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29975-1-AP (Uromodulin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 29975-1-AP

    IF Staining of mouse kidney using 29975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Uromodulin antibody (29975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse kidney using 29975-1-AP

    IF Staining of mouse kidney using 29975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Uromodulin antibody (29975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman urine sample
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29975-1-AP targets Uromodulin in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Uromodulin fusion protein Ag32343 相同性解析による交差性が予測される生物種
Full Name uromodulin
Calculated molecular weight 640 aa, 70 kDa
Observed molecular weight80-90 kDa
GenBank accession numberBC035975
Gene symbol UMOD
Gene ID (NCBI) 7369
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin has been linked to water/electrolyte balance and to kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721)

Protocols

Product Specific Protocols
WB protocol for Uromodulin antibody 29975-1-APDownload protocol
IHC protocol for Uromodulin antibody 29975-1-APDownload protocol
IF protocol for Uromodulin antibody 29975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols