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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ULK1 Polyclonal antibody

ULK1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29005-1-AP

Synonyms

ATG1, FLJ38455, FLJ46475, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 29005-1-AP

    WB analysis of HeLa using 29005-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 29005-1-AP (ULK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 29005-1-AP

    IF Staining of HeLa using 29005-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ULK1 antibody (29005-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

29005-1-AP targets ULK1 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity goat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name unc-51-like kinase 1 (C. elegans)
Observed molecular weight130 kDa
GenBank accession numberNM_003565
Gene symbol ULK1
Gene ID (NCBI) 8408
RRIDAB_2918228
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

Protocols

Product Specific Protocols
WB protocol for ULK1 antibody 29005-1-APDownload protocol
IF protocol for ULK1 antibody 29005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
goatWB

Ecotoxicol Environ Saf

Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells

Authors - Shuaiqi Han
View Article
human

Med Oncol

GNL3L promotes autophagy via regulating AMPK signaling in esophageal cancer cells

Authors - Weiting He
View Article