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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated TXN Monoclonal antibody

TXN Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F3C1

Cat no : CL594-66475

Synonyms

ADF, ATL derived factor, DKFZp686B1993, SASP, thioredoxin, TRDX, TRX, TRX1, TXN

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-66475

    IF Staining of HepG2 using CL594-66475

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated TXN antibody (CL594-66475, Clone: 1F3C1 ) at dilution of 1:1000, C488-Phalloidin (green).

  • IF Staining of HepG2 using CL594-66475

    IF Staining of HepG2 using CL594-66475

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66475 (TXN antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TXN Ready-To-Use IHC Kit. TXN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66475-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

CL594-66475 targets TXN in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TXN fusion protein Ag6355 相同性解析による交差性が予測される生物種
Full Name thioredoxin
Calculated molecular weight 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC003377
Gene symbol TXN
Gene ID (NCBI) 7295
RRIDAB_2883587
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Protocols

Product Specific Protocols
IF protocol for CL594 TXN antibody CL594-66475Download protocol
Standard Protocols
Click here to view our Standard Protocols