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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM44 Monoclonal antibody

TRIM44 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1B12H12

Cat no : 66249-1-Ig

Synonyms

DIPB, HSA249128, MC7, Protein DIPB, TRIM44, tripartite motif containing 44

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 66249-1-Ig

    WB analysis of MCF-7 using 66249-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66249-1-Ig (TRIM44 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 66249-1-Ig

    WB analysis of human testis using 66249-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66249-1-Ig (TRIM44 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 66249-1-Ig

    IF Staining of MCF-7 using 66249-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66249-1-Ig(TRIM44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, human testis tissue
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

66249-1-Ig targets TRIM44 in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TRIM44 fusion protein Ag18771 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 44
Calculated molecular weight 344 aa, 38 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC024031
Gene symbol TRIM44
Gene ID (NCBI) 54765
RRIDAB_2881637
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM44 is one of the family member of TRIM protein, which contains an N-terminal ubiquitin hydrolase-type zinc-finger domain, followed by a coiled-coil domain, a zinc-finger B-box homology domain, and a second coiled-coil domain near the C-terminus. Members of the TRIM protein family are involved in various cellular processes, such as cell proliferation, oncogenesis and antiviral defense.

Protocols

Product Specific Protocols
WB protocol for TRIM44 antibody 66249-1-IgDownload protocol
IF protocol for TRIM44 antibody 66249-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol

Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.

Authors - Zhangchuan Xia
View Article
humanWB

J Immunol

NAC1 Potentiates Cellular Antiviral Signaling by Bridging MAVS and TBK1.

Authors - Zhangchuan Xia
View Article