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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPRKB Monoclonal antibody

TPRKB Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D5B9

Cat no : 66402-1-Ig

Synonyms

CGI 121, My019, PRPK binding protein, TP53RK binding protein, TPRKB

Validation Data Gallery

Applications:
  • WB analysis using 66402-1-Ig

    WB analysis using 66402-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66402-1-Ig (TPRKB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66402-1-Ig

    WB analysis of HeLa using 66402-1-Ig

    Western blot analysis of TPRKB in various cell lines using Proteintech antibody 66402-1-Ig at a dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66402-1-Ig

    WB analysis of HEK-293 using 66402-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66402-1-Ig (TPRKB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 66402-1-Ig

    IF Staining of Jurkat using 66402-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TPRKB antibody (66402-1-Ig, Clone: 2D5B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, COLO 320 cells, Caco-2 cells, Jurkat cells, Raji cells
Positive IF detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66402-1-Ig targets TPRKB in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TPRKB fusion protein Ag18770 相同性解析による交差性が予測される生物種
Full Name TP53RK binding protein
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC029492
Gene symbol TPRKB
Gene ID (NCBI) 51002
RRIDAB_2881776
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPRKB, also named as CGI 121, belongs to the CGI121/TPRKB family. It interacts with TP53RK/PRPK and is widely expressed in all tissues.

Protocols

Product Specific Protocols
WB protocol for TPRKB antibody 66402-1-IgDownload protocol
IF protocol for TPRKB antibody 66402-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols