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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNF Beta Polyclonal antibody

TNF Beta Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13111-1-AP

Synonyms

LT, LT alpha, LTA, Lymphotoxin alpha, TNF beta, TNFB, TNF-beta, TNFSF1

Validation Data Gallery

Applications:
  • IHC staining of human malignant melanoma using 13111-1-AP

    IHC staining of human malignant melanoma using 13111-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13111-1-AP (TNF beta antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human malignant melanoma using 13111-1-AP

    IHC staining of human malignant melanoma using 13111-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13111-1-AP (TNF beta antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 13111-1-AP

    IHC staining of human tonsillitis using 13111-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13111-1-AP (TNF beta antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 13111-1-AP

    IHC staining of human tonsillitis using 13111-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13111-1-AP (TNF beta antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 13111-1-AP

    IHC staining of human testis using 13111-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13111-1-AP (TNF beta antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 13111-1-AP

    IHC staining of human testis using 13111-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13111-1-AP (TNF beta antibody at dilution of 1:200 (under 40x lens).

Tested Applications

Positive IHC detected inhuman malignant melanoma tissue, human testis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13111-1-AP targets TNF Beta in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNF Beta fusion protein Ag3775 相同性解析による交差性が予測される生物種
Full Name lymphotoxin alpha (TNF superfamily, member 1)
Calculated molecular weight 205 aa, 22 kDa
GenBank accession numberBC034729
Gene symbol LTA
Gene ID (NCBI) 4049
RRIDAB_2877916
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for TNF Beta antibody 13111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Diagnostics (Basel)

Loss of Lymphotoxin Alpha-Expressing Memory B Cells Correlates with Metastasis of Human Primary Melanoma.

Authors - Franziska Werner
View Article
mouseWB

Phytomedicine

Glycosides from Buyang Huanwu Decoction inhibit atherosclerotic inflammation via JAK/STAT signaling pathway

Authors - Xinying Fu
View Article