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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNC/Tenascin-C Polyclonal antibody

TNC/Tenascin-C Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27789-1-AP

Synonyms

Cytotactin, GMEM, GP 150 225, Hexabrachion, HXB, JI, Myotendinous antigen, Neuronectin, Tenascin, tenascin C, TN, TN C, TNC

Validation Data Gallery

Applications:
  • WB analysis using 27789-1-AP

    WB analysis using 27789-1-AP

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 27789-1-AP (TNC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27789-1-AP

    IHC staining of human liver cancer using 27789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 27789-1-AP

    IHC staining of human liver cancer using 27789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 27789-1-AP

    IHC staining of human lung cancer using 27789-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 27789-1-AP

    IHC staining of human lung cancer using 27789-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 27789-1-AP

    IHC staining of human breast cancer using 27789-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 27789-1-AP

    IHC staining of human breast cancer using 27789-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inU-87 MG cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

27789-1-AP targets TNC/Tenascin-C in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNC/Tenascin-C fusion protein Ag27122 相同性解析による交差性が予測される生物種
Full Name tenascin C
Calculated molecular weight 2201 aa, 241 kDa
GenBank accession numberBC151843
Gene symbol TNC
Gene ID (NCBI) 3371
RRIDAB_2918131
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.

Protocols

Product Specific Protocols
WB protocol for TNC/Tenascin-C antibody 27789-1-APDownload protocol
IHC protocol for TNC/Tenascin-C antibody 27789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols