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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated TMF1 Monoclonal antibody

TMF1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G12F2

Cat no : CL594-67505

Synonyms

ARA160, TATA element modulatory factor, TMF, TMF1

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-67505

    IF Staining of HepG2 using CL594-67505

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 TMF1 antibody (CL594-67505, Clone: 3G12F2 ) at dilution of 1:200, CL488-Phalloidin (green).

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TMF1 Ready-To-Use IHC Kit. TMF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67505-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67505 targets TMF1 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMF1 fusion protein Ag17666 相同性解析による交差性が予測される生物種
Full Name TATA element modulatory factor 1
Calculated molecular weight 1093 aa, 123 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberBC126123
Gene symbol TMF1
Gene ID (NCBI) 7110
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.

Protocols

Product Specific Protocols
IF protocol for CL594 TMF1 antibody CL594-67505Download protocol
Standard Protocols
Click here to view our Standard Protocols