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  • Featured Product
  • KD/KO Validated

TMF1 Monoclonal antibody

TMF1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G12F2

Cat no : 67505-1-Ig

Synonyms

ARA160, TATA element modulatory factor, TMF, TMF1

Validation Data Gallery

Applications:
  • WB analysis using 67505-1-Ig

    WB analysis using 67505-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67505-1-Ig (TMF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67505-1-Ig

    WB analysis of HeLa using 67505-1-Ig

    WB result of TMF1 antibody (67505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMF1 transfected HeLa cells.

  • IF Staining of HepG2 using 67505-1-Ig

    IF Staining of HepG2 using 67505-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67505-1-Ig (TMF1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TMF1 Ready-To-Use IHC Kit. TMF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67505-1-Ig targets TMF1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMF1 fusion protein Ag17666 相同性解析による交差性が予測される生物種
Full Name TATA element modulatory factor 1
Calculated molecular weight 1093 aa, 123 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberBC126123
Gene symbol TMF1
Gene ID (NCBI) 7110
RRIDAB_2882728
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for TMF1 antibody 67505-1-IgDownload protocol
IF protocol for TMF1 antibody 67505-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (08-03-2022)

Very good signal in WB; a bit non specific staining in IF

  • Applications: Western Blot, Immunofluorescence